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Selleck Chemicals mcc950 sodium

Experimental design. A total of 82 mice (male 42 and female 40) of both HD and WT were treated with <t>MCC950</t> solution or vehicle (drinking water) from 6 to 20 weeks of age. Depression and anxiety‐like behaviour was evaluated at week 10 and 11. Cognitive function was assessed from 12 to 13 weeks of age. Motor function was evaluated every week by clasping score and rotarpd performance. At week 14, gait was assessed to further evaluate the motor function by digigait analysis. Gut function was evaluated at week 15 and 20 by GTT and faecal output and water content measurement. At week 20, gut permeability and gut macroscopy were assessed. Abbreviations: GTT, gut transit time; HD, Huntington's disease; MWM, Morris water maze; NOR, novel object recognition; NSFT, novelty‐suppressed feeding test; WT, wild type. Figure created in Biorender.com .

Mcc950 Sodium, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 95/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mcc950+sodium/MCC950+Sodium/pmc13033966-71-15-17
Average 95 stars, based on 80 article reviews

mcc950 sodium - by Bioz Stars, 2026-11

95/100 stars

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1) Product Images from "Inhibition of the NLRP3 Inflammasome With MCC950 Improves Gut Health in Huntington's Disease Mice"

Article Title: Inhibition of the NLRP3 Inflammasome With MCC950 Improves Gut Health in Huntington's Disease Mice

Journal: Journal of Neurochemistry

doi: 10.1111/jnc.70419

Experimental design. A total of 82 mice (male 42 and female 40) of both HD and WT were treated with MCC950 solution or vehicle (drinking water) from 6 to 20 weeks of age. Depression and anxiety‐like behaviour was evaluated at week 10 and 11. Cognitive function was assessed from 12 to 13 weeks of age. Motor function was evaluated every week by clasping score and rotarpd performance. At week 14, gait was assessed to further evaluate the motor function by digigait analysis. Gut function was evaluated at week 15 and 20 by GTT and faecal output and water content measurement. At week 20, gut permeability and gut macroscopy were assessed. Abbreviations: GTT, gut transit time; HD, Huntington's disease; MWM, Morris water maze; NOR, novel object recognition; NSFT, novelty‐suppressed feeding test; WT, wild type. Figure created in Biorender.com .

Figure Legend Snippet: Experimental design. A total of 82 mice (male 42 and female 40) of both HD and WT were treated with MCC950 solution or vehicle (drinking water) from 6 to 20 weeks of age. Depression and anxiety‐like behaviour was evaluated at week 10 and 11. Cognitive function was assessed from 12 to 13 weeks of age. Motor function was evaluated every week by clasping score and rotarpd performance. At week 14, gait was assessed to further evaluate the motor function by digigait analysis. Gut function was evaluated at week 15 and 20 by GTT and faecal output and water content measurement. At week 20, gut permeability and gut macroscopy were assessed. Abbreviations: GTT, gut transit time; HD, Huntington's disease; MWM, Morris water maze; NOR, novel object recognition; NSFT, novelty‐suppressed feeding test; WT, wild type. Figure created in Biorender.com .

Techniques Used: Permeability

Effects of MCC950 on phenotypic features of HD and WT littermate control mice. Body weight (a, b), food intake (c, d), fluid intake (e, f), brain weight (g, h). Data are displayed as mean ± SEM. Statistical analyses were performed using linear mixed model repeated measure analysis/generalised LMM repeated measure analysis (a–f) and linear mixed model (LMM)/generalised LMM (g–h) followed by Bonferroni post hoc adjustment with emmeans package in R . p value ( α ) = 0.05. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05. n = 10–11 (male), n = 10 (female). Symbols in Figure , indicate the significance of the post hoc comparisons as follows: *HD (H2O vs MCC), #WT (H2O vs MCC), @H2O (WT vs HD), $MCC (WT vs HD) and for each comparsion one to four symbols in the graph represent p < 0.05 to p < 0.0001, respectively. HD, Huntington's disease; MCC, MCC950; WT, wild type.

Figure Legend Snippet: Effects of MCC950 on phenotypic features of HD and WT littermate control mice. Body weight (a, b), food intake (c, d), fluid intake (e, f), brain weight (g, h). Data are displayed as mean ± SEM. Statistical analyses were performed using linear mixed model repeated measure analysis/generalised LMM repeated measure analysis (a–f) and linear mixed model (LMM)/generalised LMM (g–h) followed by Bonferroni post hoc adjustment with emmeans package in R . p value ( α ) = 0.05. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05. n = 10–11 (male), n = 10 (female). Symbols in Figure , indicate the significance of the post hoc comparisons as follows: *HD (H2O vs MCC), #WT (H2O vs MCC), @H2O (WT vs HD), $MCC (WT vs HD) and for each comparsion one to four symbols in the graph represent p < 0.05 to p < 0.0001, respectively. HD, Huntington's disease; MCC, MCC950; WT, wild type.

Techniques Used: Control

Effects of MCC950 on motor function of HD and WT littermate control. Clasping score (a, b), rotarod performance (c, d), digigait (e, f). Data are displayed as mean ± SEM. Statistical analyses were performed using CLMMs (cumulative link mixed models) (a‐b), linear mixed model repeated measure analysis/generalised LMM repeated measure analysis (c, d), and linear mixed model (LMM)/generalised LMM (e, f) followed by Bonferroni post hoc adjustment with emmeans package in R. p value ( α ) = 0.05 and **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns= non‐significant n = 10–11 (male), n = 10 (female) for clasping score and rotarod; n = 8–10 (male), n = 9–10 (female) for digigait. Symbols in Figure , indicate the significance of post hoc comparisons as follows: *H2O (HD vs WT), #WT (H2O vs MCC950), $HD (H2O vs MCC950). Also, in Figure and , # symbol represents the significant interaction between treatment and age. One to four symbols in the graph represent p < 0.05 to p < 0.0001, respectively. HD, Huntington's disease; WT, wild type.

Figure Legend Snippet: Effects of MCC950 on motor function of HD and WT littermate control. Clasping score (a, b), rotarod performance (c, d), digigait (e, f). Data are displayed as mean ± SEM. Statistical analyses were performed using CLMMs (cumulative link mixed models) (a‐b), linear mixed model repeated measure analysis/generalised LMM repeated measure analysis (c, d), and linear mixed model (LMM)/generalised LMM (e, f) followed by Bonferroni post hoc adjustment with emmeans package in R. p value ( α ) = 0.05 and **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns= non‐significant n = 10–11 (male), n = 10 (female) for clasping score and rotarod; n = 8–10 (male), n = 9–10 (female) for digigait. Symbols in Figure , indicate the significance of post hoc comparisons as follows: *H2O (HD vs WT), #WT (H2O vs MCC950), $HD (H2O vs MCC950). Also, in Figure and , # symbol represents the significant interaction between treatment and age. One to four symbols in the graph represent p < 0.05 to p < 0.0001, respectively. HD, Huntington's disease; WT, wild type.

Techniques Used: Control

Effects of MCC950 on short‐term memory and learning of HD and WT littermate control mice. Novel object recognition (NOR) test (a–c), Y‐maze test (d–g). Data are displayed as mean ± SEM. Statistical analyses were performed using linear mixed model (LMM)/generalised LMM (a–c), generalised linear model (d) and linear mixed model/generalised LMM repeated measure analysis (c, f, g) followed by Bonferroni post hoc adjustment with emmeans package in R. p value ( α ) = 0.05 and **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = non‐significant. n = 10–11 (male), n = 10 (female). HD, Huntington's disease; WT, wild type.

Figure Legend Snippet: Effects of MCC950 on short‐term memory and learning of HD and WT littermate control mice. Novel object recognition (NOR) test (a–c), Y‐maze test (d–g). Data are displayed as mean ± SEM. Statistical analyses were performed using linear mixed model (LMM)/generalised LMM (a–c), generalised linear model (d) and linear mixed model/generalised LMM repeated measure analysis (c, f, g) followed by Bonferroni post hoc adjustment with emmeans package in R. p value ( α ) = 0.05 and **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = non‐significant. n = 10–11 (male), n = 10 (female). HD, Huntington's disease; WT, wild type.

Techniques Used: Control

Effects of MCC950 on longer‐term associative cognition and spatial memory of HD and WT littermate control mice (CFC test). Fear conditioning (a, b) and fear extinction (c, d). Data are displayed as mean ± SEM. Statistical analyses were performed using linear mixed model repeated measure analysis/generalised LMM repeated measure analysis (a–d) followed by Bonferroni post hoc adjustment with emmeans package in R. p ‐value ( α ) = 0.05 and ####,**** p < 0.0001, ###,*** p < 0.001, ##,** p < 0.01, #,* p < 0.05. n = 10–11 (male), n = 10 (female). HD, Huntington's disease; MCC, MCC950; WT, wild type.

Figure Legend Snippet: Effects of MCC950 on longer‐term associative cognition and spatial memory of HD and WT littermate control mice (CFC test). Fear conditioning (a, b) and fear extinction (c, d). Data are displayed as mean ± SEM. Statistical analyses were performed using linear mixed model repeated measure analysis/generalised LMM repeated measure analysis (a–d) followed by Bonferroni post hoc adjustment with emmeans package in R. p ‐value ( α ) = 0.05 and ####,**** p < 0.0001, ###,*** p < 0.001, ##,** p < 0.01, #,* p < 0.05. n = 10–11 (male), n = 10 (female). HD, Huntington's disease; MCC, MCC950; WT, wild type.

Techniques Used: Control

Effects of MCC950 on longer‐term associative cognition and spatial memory of HD and WT littermate control mice (MWM). Latency to reach the platform during training period of MWM test (a, b), distance travelled to reach the platform during training period of MWM test (c, d), time spent in each quadrant on day 6 of MWM test (e, f), latency to reach the platform on day 6 of MWM test (g), times spent in target quadrant on day 6 of MWM test (h), total distance travelled on day 6 of MWM test (i), velocity on day 6 of MWM test (j). Data are displayed as mean ± SEM. Statistical analyses were performed using linear mixed model repeated measure analysis/generalised LMM repeated measure analysis (a–f) and linear mixed model (LMM)/generalised LMM (g–j) followed by Bonferroni post hoc adjustment with emmeans package in R. p value ( α ) = 0.05 and ####,**** p < 0.0001, ###,*** p < 0.001, ##,** p < 0.01, #,* p < 0.05, ns= non‐significant. n = 8–11 (male), n = 7–10 (female). HD, Huntington's disease; MWM, Morris water maze; NE, northeast; NW, northwest; SE, southeast (target quadrant); SW, southwest; WT, wild type.

Figure Legend Snippet: Effects of MCC950 on longer‐term associative cognition and spatial memory of HD and WT littermate control mice (MWM). Latency to reach the platform during training period of MWM test (a, b), distance travelled to reach the platform during training period of MWM test (c, d), time spent in each quadrant on day 6 of MWM test (e, f), latency to reach the platform on day 6 of MWM test (g), times spent in target quadrant on day 6 of MWM test (h), total distance travelled on day 6 of MWM test (i), velocity on day 6 of MWM test (j). Data are displayed as mean ± SEM. Statistical analyses were performed using linear mixed model repeated measure analysis/generalised LMM repeated measure analysis (a–f) and linear mixed model (LMM)/generalised LMM (g–j) followed by Bonferroni post hoc adjustment with emmeans package in R. p value ( α ) = 0.05 and ####,**** p < 0.0001, ###,*** p < 0.001, ##,** p < 0.01, #,* p < 0.05, ns= non‐significant. n = 8–11 (male), n = 7–10 (female). HD, Huntington's disease; MWM, Morris water maze; NE, northeast; NW, northwest; SE, southeast (target quadrant); SW, southwest; WT, wild type.

Techniques Used: Control

Figure Legend Snippet: Effects of MCC950 on the gut profile of HD and WT littermate control mice. Faecal water content (a, b), faecal consistency (Bristol stool score) (c, d), faecal output (e, f), gut transit time (GTT) (g, h), gut permeability (i, j). Data are displayed as mean ± SEM. Statistical analyses were performed using linear mixed model repeated measure analysis/generalised LMM repeated measure analysis (a, b, e–h), CLMMs (cumulative link mixed models) (c, d), linear mixed model (LMM)/generalised LMM (i, j) followed by Bonferroni post hoc adjustment with emmeans package in R. p ‐value ( α ) = 0.05 and ####,**** p < 0.0001, ###,*** p < 0.001, ##,** p < 0.01, #,* p < 0.05. n = 10–11 (male), n = 10 (female). HD, Huntington's disease; GTT, gut transit time; WT, wild type.

Techniques Used: Control, Permeability

Effects of MCC950 on gut macroscopy of HD and WT littermate control mice. Cecal length (a, b), cecal weight (c, d), colon length (e, f), colon weight (g, h), colon weight to length ratio (i). Data are displayed as mean ± SEM. Statistical analyses were performed using linear mixed model (LMM)/generalised LMM followed by Bonferroni post hoc adjustment with emmeans package in R. p value ( α ) = 0.05 and **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = non‐significant. n = 9–11 (male), n = 9–10 (female). HD, Huntington's disease; WT, wild type.

Figure Legend Snippet: Effects of MCC950 on gut macroscopy of HD and WT littermate control mice. Cecal length (a, b), cecal weight (c, d), colon length (e, f), colon weight (g, h), colon weight to length ratio (i). Data are displayed as mean ± SEM. Statistical analyses were performed using linear mixed model (LMM)/generalised LMM followed by Bonferroni post hoc adjustment with emmeans package in R. p value ( α ) = 0.05 and **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = non‐significant. n = 9–11 (male), n = 9–10 (female). HD, Huntington's disease; WT, wild type.

Techniques Used: Control

Effect of MCC950 on gut microbiome diversity of HD and WT littermate control mice. Comparison of community (Shannon alpha diversity) of gut microbiome stratified by sex under MCC950 or water for both WT and HD mice (a). PCA plot of microbial ASV based on Aitchison distance for Beta diversity (b). n = 8 (male), n = 8 (female). Box plots show the median (central line) and interquartile range (box); whiskers represent 1.5× the interquartile range; points represent individual animals. ASV, amplicon sequence variant; HD, Huntington's disease; PCA, principal component analysis; WT, wild type.

Figure Legend Snippet: Effect of MCC950 on gut microbiome diversity of HD and WT littermate control mice. Comparison of community (Shannon alpha diversity) of gut microbiome stratified by sex under MCC950 or water for both WT and HD mice (a). PCA plot of microbial ASV based on Aitchison distance for Beta diversity (b). n = 8 (male), n = 8 (female). Box plots show the median (central line) and interquartile range (box); whiskers represent 1.5× the interquartile range; points represent individual animals. ASV, amplicon sequence variant; HD, Huntington's disease; PCA, principal component analysis; WT, wild type.

Techniques Used: Control, Comparison, Amplification, Sequencing, Variant Assay

Effect of MCC950 on NLRP3, IL‐1β, Caspase‐1 expression in gut (proximal colon) of HD and WT littermate control mice. NLRP3 and IL‐1β (a), caspase‐1 (b, c). Data are displayed as mean ± SEM. Statistical analyses were performed using linear model (LM)/generalised LM followed by Bonferroni post hoc adjustment with emmeans package in R. p value ( α ) = 0.05 and **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = non‐significant. n = 5–7 (2 blots for males and 2 blots for females). After transferring the protein from gel to membrane, ponceau S staining was performed and then each membrane was split into two parts based on the size of the protein of interest. Upper part of the membrane was probed with NLRP3 antibody. While lower part of the membrane was first probed with IL‐1β followed by caspase‐1. Membrane was stripped using stripping buffer in between two experiments of IL‐1β and caspase‐1 detection. Representative image of ponceau S staining as loading control for both NLRP3 and IL‐1β (a), and caspase‐1 (b) is shown in NLRP3 and IL‐1β (a) panel only as it was derived from a single membrane and image was captured before probing with any antibody. Positive controls, poly I:C (spleen), BMDM: LPS + Nig. BMDM, Bone‐marrow‐derived macrophage; Cas 1 KO, caspase‐1 knock out; HD H 2 O, Huntington's disease water; HD MC, Huntington's disease MCC950, NL KO, NLRP3 knock out; IL‐1beta KO, Interleukin 1 beta knock out; IL‐1β/IL‐1beta, Interleukin 1 beta; LPS, Lipopolysaccharides; Nig, nigericin; WT H 2 O, wild type water; WT MC, wild type MCC950.

Figure Legend Snippet: Effect of MCC950 on NLRP3, IL‐1β, Caspase‐1 expression in gut (proximal colon) of HD and WT littermate control mice. NLRP3 and IL‐1β (a), caspase‐1 (b, c). Data are displayed as mean ± SEM. Statistical analyses were performed using linear model (LM)/generalised LM followed by Bonferroni post hoc adjustment with emmeans package in R. p value ( α ) = 0.05 and **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = non‐significant. n = 5–7 (2 blots for males and 2 blots for females). After transferring the protein from gel to membrane, ponceau S staining was performed and then each membrane was split into two parts based on the size of the protein of interest. Upper part of the membrane was probed with NLRP3 antibody. While lower part of the membrane was first probed with IL‐1β followed by caspase‐1. Membrane was stripped using stripping buffer in between two experiments of IL‐1β and caspase‐1 detection. Representative image of ponceau S staining as loading control for both NLRP3 and IL‐1β (a), and caspase‐1 (b) is shown in NLRP3 and IL‐1β (a) panel only as it was derived from a single membrane and image was captured before probing with any antibody. Positive controls, poly I:C (spleen), BMDM: LPS + Nig. BMDM, Bone‐marrow‐derived macrophage; Cas 1 KO, caspase‐1 knock out; HD H 2 O, Huntington's disease water; HD MC, Huntington's disease MCC950, NL KO, NLRP3 knock out; IL‐1beta KO, Interleukin 1 beta knock out; IL‐1β/IL‐1beta, Interleukin 1 beta; LPS, Lipopolysaccharides; Nig, nigericin; WT H 2 O, wild type water; WT MC, wild type MCC950.

Techniques Used: Expressing, Control, Transferring, Membrane, Staining, Stripping Membranes, Derivative Assay, Knock-Out

other:

Article Title: Effects of acute pro-inflammatory stimulation and 25-hydroxycholesterol on hippocampal plasticity and learning involve NLRP3 inflammasome and cellular stress responses.
Article Snippet: MCC950 sodium (CAS 256373-96-3, Cat# S7809) and BAL-0028 (CAS 2842012-69-3, Cat# E1970) were purchased from Selleck Chemicals (Houston TX).

Article Title: Effects of acute pro-inflammatory stimulation and 25-hydroxycholesterol on hippocampal plasticity and learning involve NLRP3 inflammasome and cellular stress responses
Article Snippet: MCC950 sodium (CAS 256373-96-3, Cat# S7809) and BAL-0028 (CAS 2842012-69-3, Cat# E1970) were purchased from Selleck Chemicals (Houston TX).

Activation Assay:

Article Title: Transient Dietary Changes Modulate Inflammatory Disease Trajectory in the Lean State
Article Snippet: .. To inhibit NLRP3 activation, MCC950 sodium (#S7809, Selleckchem) was prepared in 50 mg mL −1 in water and 75 μg was given topically to each mouse daily in sync with application of IMQ. ..

Image Search Results

FAP α + HSCs upregulate NF- κ B/NLRP3/caspase-1/IL-18 signaling pathway. (A) KEGG analysis of the Top100 upregulated genes in LX-2 FAP cells. (B) GO analysis of paracancerous and healthy HSCs from scRNA sequencing. (C) Western blotting analysis of FAP α , NF κ B-p65, p- NF κ B-p65, NLRP3, pro caspase-1, caspase-1 P 20, IL-18 in LX-2 FAP cells with or without SP-13786 ( n = 3). (D) Immunocytochemistry of NLRP3 in LX-2 FAP cells with or without SP-13786 ( n = 3). Scale bar, 10 μm. (E) ELISA analysis of IL-18 concentration in LX-2 FAP cells ( n = 3). (F) ELISA analysis of IL-18 in the peripheral blood of Fap fl / fl or Fap ΔGfap mice with MC38-luc or Panc02-luc injection ( n = 5). (G, H) Immunofluorescence staining of NLRP3, caspase-1, or IL-18 (green) in α SMA + HSCs (gray) that adhered to the CD31 + sinusoidal blood vessels (red) in the PMN of CRC or pancreatic cancer ( n = 5). Scale bar, 10 μm. Data are presented as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 (one-way ANOVA with Tukey's post hoc comparison in D; two-tailed unpaired t -test in E–H).

Journal: Acta Pharmaceutica Sinica. B

Article Title: Targeting FAP α -positive hepatic stellate cells ameliorates the formation of pre-metastatic niche

doi: 10.1016/j.apsb.2026.04.018

Figure Lengend Snippet: FAP α + HSCs upregulate NF- κ B/NLRP3/caspase-1/IL-18 signaling pathway. (A) KEGG analysis of the Top100 upregulated genes in LX-2 FAP cells. (B) GO analysis of paracancerous and healthy HSCs from scRNA sequencing. (C) Western blotting analysis of FAP α , NF κ B-p65, p- NF κ B-p65, NLRP3, pro caspase-1, caspase-1 P 20, IL-18 in LX-2 FAP cells with or without SP-13786 ( n = 3). (D) Immunocytochemistry of NLRP3 in LX-2 FAP cells with or without SP-13786 ( n = 3). Scale bar, 10 μm. (E) ELISA analysis of IL-18 concentration in LX-2 FAP cells ( n = 3). (F) ELISA analysis of IL-18 in the peripheral blood of Fap fl / fl or Fap ΔGfap mice with MC38-luc or Panc02-luc injection ( n = 5). (G, H) Immunofluorescence staining of NLRP3, caspase-1, or IL-18 (green) in α SMA + HSCs (gray) that adhered to the CD31 + sinusoidal blood vessels (red) in the PMN of CRC or pancreatic cancer ( n = 5). Scale bar, 10 μm. Data are presented as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 (one-way ANOVA with Tukey's post hoc comparison in D; two-tailed unpaired t -test in E–H).

Article Snippet: LX-2 Vector and LX-2 FAP cells were stimulated with an NLRP3 inhibitor MCC950 (1 μmol/L, T6887, TargetMol) for 1 h. THP-1 cells were differentiated to M φ by phorbol 12-myristate 13-acetate (PMA, 200 nmol/L, TQ0198, TargetMol) for 16 h.

Techniques: Analysis, Sequencing, Western Blot, Immunocytochemistry, Enzyme-linked Immunosorbent Assay, Concentration Assay, Injection, Immunofluorescence, Staining, Comparison, Two Tailed Test, Significance Assay

FAP α + HSCs induce ECM remodeling and macrophage infiltration via NLRP3. (A) The spleen injection of MC38-luc cells into AAV-shCTL or AAV-sh Nlrp3 mice. (B) Masson staining in the PMN of the CRC or pancreatic cancer in AAV-shCTL or AAV-sh Nlrp3 mice ( n = 5). Scale bar, 50 μm. (C) Immunofluorescence staining and quantification of COL1A1 and Fibronectin in the PMN of AAV-shCTL or AAV-sh Nlrp3 mice ( n = 5). Scale bar, 20 μm. (D) Immunofluorescence staining and quantification of CD206 + macrophages in the PMN of AAV-shCTL or AAV-sh Nlrp3 mice ( n = 5). Scale bar, 20 μm. (E) Statistical analysis of immunocytochemistry of COL1A1 and Fibronectin in LX-2 FAP cells following knockdown of NLRP1, NLRP3, NLRC4, or AIM2 ( n = 3). (F) Transwell assays for the migration and invasion of M ϕ co-cultured with LX-2 FAP cells following knockdown of NLRP1, NLRP3, NLRC4, or AIM2 were added to the lower chambers of transwell plates ( n = 3). (G) RT-qPCR analysis of MRC1, ARG1, CCL17, NOS2, TNF, IL6 in M ϕ cultured with CM from LX-2 FAP cells with or without NLRP3-knockdown ( n = 3). (H) Western blotting analysis of FAP α , NLRP3, pro caspase-1, caspase-1 P 20, IL-18 in LX-2 FAP cells treated with or without NLRP3-knockdown ( n = 3). (I) ELISA analysis of IL-18 in LX-2 FAP cells processed through NLRP3-knockdown or not ( n = 3). (J) Mechanical diagram of FAP α + HSCs inducing ECM remodeling and macrophage infiltration. Data are presented as mean ± SEM. ns, no significance; ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 (two-tailed unpaired t -test in B–D; one-way ANOVA with Tukey's post hoc comparison in E–G, I).

Journal: Acta Pharmaceutica Sinica. B

Article Title: Targeting FAP α -positive hepatic stellate cells ameliorates the formation of pre-metastatic niche

doi: 10.1016/j.apsb.2026.04.018

Figure Lengend Snippet: FAP α + HSCs induce ECM remodeling and macrophage infiltration via NLRP3. (A) The spleen injection of MC38-luc cells into AAV-shCTL or AAV-sh Nlrp3 mice. (B) Masson staining in the PMN of the CRC or pancreatic cancer in AAV-shCTL or AAV-sh Nlrp3 mice ( n = 5). Scale bar, 50 μm. (C) Immunofluorescence staining and quantification of COL1A1 and Fibronectin in the PMN of AAV-shCTL or AAV-sh Nlrp3 mice ( n = 5). Scale bar, 20 μm. (D) Immunofluorescence staining and quantification of CD206 + macrophages in the PMN of AAV-shCTL or AAV-sh Nlrp3 mice ( n = 5). Scale bar, 20 μm. (E) Statistical analysis of immunocytochemistry of COL1A1 and Fibronectin in LX-2 FAP cells following knockdown of NLRP1, NLRP3, NLRC4, or AIM2 ( n = 3). (F) Transwell assays for the migration and invasion of M ϕ co-cultured with LX-2 FAP cells following knockdown of NLRP1, NLRP3, NLRC4, or AIM2 were added to the lower chambers of transwell plates ( n = 3). (G) RT-qPCR analysis of MRC1, ARG1, CCL17, NOS2, TNF, IL6 in M ϕ cultured with CM from LX-2 FAP cells with or without NLRP3-knockdown ( n = 3). (H) Western blotting analysis of FAP α , NLRP3, pro caspase-1, caspase-1 P 20, IL-18 in LX-2 FAP cells treated with or without NLRP3-knockdown ( n = 3). (I) ELISA analysis of IL-18 in LX-2 FAP cells processed through NLRP3-knockdown or not ( n = 3). (J) Mechanical diagram of FAP α + HSCs inducing ECM remodeling and macrophage infiltration. Data are presented as mean ± SEM. ns, no significance; ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 (two-tailed unpaired t -test in B–D; one-way ANOVA with Tukey's post hoc comparison in E–G, I).

Techniques: Injection, Staining, Immunofluorescence, Analysis, Immunocytochemistry, Knockdown, Migration, Cell Culture, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Comparison

Targeting FAP α + HSCs to inhibit the formation of liver PMN and reduce CRCLM. (A) Therapeutic schedules for Z-GP-DAVLBH (2 mg/kg) treatment in mice bearing HCT116 cells. Livers in the period of PMN were attained for pathological analysis and tumors were harvested and photographed. (B) Masson staining in the PMN of the CRC ( n = 5). Scale bar, 50 μm. (C) Immunofluorescence staining and quantification of COL1A1 and Fibronectin in the PMN of the CRC ( n = 5). Scale bar, 20 μm. (D) Immunofluorescence staining and quantification of F4/80 + or Gr-1 + cells in the pre-metastatic niche of the CRC after Z-GP-DAVLBH treatments ( n = 5). Scale bar, 20 μm. (E) Immunofluorescence staining of the α SMA + HSCs (green) that adhered the CD31 + sinusoidal blood vessels (red) in the pre-metastatic niche of the CRC after Z-GP-DAVLBH treatments. Scale bar, 20 μm. Quantification of the activated HSCs is shown ( n = 5). (F) Immunofluorescence staining of NLRP3, caspase-1 or IL-18 (green) in α SMA + HSCs (gray) that adhered the CD31 + sinusoidal blood vessels (red) in the PMN of the CRC ( n = 5). Scale bar, 10 μm. (G) ELISA analysis of IL-18 in the PMN of the CRC after Z-GP-DAVLBH treatments ( n = 5). (H) Representative images of bioluminescence signals in the CRC livers after Z-GP-DAVLBH treatments ( n = 5). (I) Representative images and analysis of liver foci from HCT116-luc-injecting mice ( n = 5). Scale bar, 5 mm (top) and 2 mm (bottom). (J) The overall survival curves of mice bearing HCT116-injection treated with vehicle and Z-GP-DAVLBH ( n = 5). Data are presented as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 b y two-tailed unpaired t -test.

Journal: Acta Pharmaceutica Sinica. B

Article Title: Targeting FAP α -positive hepatic stellate cells ameliorates the formation of pre-metastatic niche

doi: 10.1016/j.apsb.2026.04.018

Figure Lengend Snippet: Targeting FAP α + HSCs to inhibit the formation of liver PMN and reduce CRCLM. (A) Therapeutic schedules for Z-GP-DAVLBH (2 mg/kg) treatment in mice bearing HCT116 cells. Livers in the period of PMN were attained for pathological analysis and tumors were harvested and photographed. (B) Masson staining in the PMN of the CRC ( n = 5). Scale bar, 50 μm. (C) Immunofluorescence staining and quantification of COL1A1 and Fibronectin in the PMN of the CRC ( n = 5). Scale bar, 20 μm. (D) Immunofluorescence staining and quantification of F4/80 + or Gr-1 + cells in the pre-metastatic niche of the CRC after Z-GP-DAVLBH treatments ( n = 5). Scale bar, 20 μm. (E) Immunofluorescence staining of the α SMA + HSCs (green) that adhered the CD31 + sinusoidal blood vessels (red) in the pre-metastatic niche of the CRC after Z-GP-DAVLBH treatments. Scale bar, 20 μm. Quantification of the activated HSCs is shown ( n = 5). (F) Immunofluorescence staining of NLRP3, caspase-1 or IL-18 (green) in α SMA + HSCs (gray) that adhered the CD31 + sinusoidal blood vessels (red) in the PMN of the CRC ( n = 5). Scale bar, 10 μm. (G) ELISA analysis of IL-18 in the PMN of the CRC after Z-GP-DAVLBH treatments ( n = 5). (H) Representative images of bioluminescence signals in the CRC livers after Z-GP-DAVLBH treatments ( n = 5). (I) Representative images and analysis of liver foci from HCT116-luc-injecting mice ( n = 5). Scale bar, 5 mm (top) and 2 mm (bottom). (J) The overall survival curves of mice bearing HCT116-injection treated with vehicle and Z-GP-DAVLBH ( n = 5). Data are presented as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 b y two-tailed unpaired t -test.

Techniques: Analysis, Staining, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Injection, Two Tailed Test

Journal: Journal of Neurochemistry

Article Title: Inhibition of the NLRP3 Inflammasome With MCC950 Improves Gut Health in Huntington's Disease Mice

doi: 10.1111/jnc.70419

Figure Lengend Snippet: Experimental design. A total of 82 mice (male 42 and female 40) of both HD and WT were treated with MCC950 solution or vehicle (drinking water) from 6 to 20 weeks of age. Depression and anxiety‐like behaviour was evaluated at week 10 and 11. Cognitive function was assessed from 12 to 13 weeks of age. Motor function was evaluated every week by clasping score and rotarpd performance. At week 14, gait was assessed to further evaluate the motor function by digigait analysis. Gut function was evaluated at week 15 and 20 by GTT and faecal output and water content measurement. At week 20, gut permeability and gut macroscopy were assessed. Abbreviations: GTT, gut transit time; HD, Huntington's disease; MWM, Morris water maze; NOR, novel object recognition; NSFT, novelty‐suppressed feeding test; WT, wild type. Figure created in Biorender.com .

Article Snippet: Starting from 6 weeks of age and up to 20 weeks, mice were treated with MCC950 sodium (Selleck Chemicals, Catalogue No. S7809) (delivered in drinking water) versus vehicle (drinking water only) ( n = 10–11 per group).

Techniques: Permeability

Journal: Journal of Neurochemistry

Article Title: Inhibition of the NLRP3 Inflammasome With MCC950 Improves Gut Health in Huntington's Disease Mice

doi: 10.1111/jnc.70419

Figure Lengend Snippet: Effects of MCC950 on phenotypic features of HD and WT littermate control mice. Body weight (a, b), food intake (c, d), fluid intake (e, f), brain weight (g, h). Data are displayed as mean ± SEM. Statistical analyses were performed using linear mixed model repeated measure analysis/generalised LMM repeated measure analysis (a–f) and linear mixed model (LMM)/generalised LMM (g–h) followed by Bonferroni post hoc adjustment with emmeans package in R . p value ( α ) = 0.05. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05. n = 10–11 (male), n = 10 (female). Symbols in Figure , indicate the significance of the post hoc comparisons as follows: *HD (H2O vs MCC), #WT (H2O vs MCC), @H2O (WT vs HD), $MCC (WT vs HD) and for each comparsion one to four symbols in the graph represent p < 0.05 to p < 0.0001, respectively. HD, Huntington's disease; MCC, MCC950; WT, wild type.

Techniques: Control

Journal: Journal of Neurochemistry

Article Title: Inhibition of the NLRP3 Inflammasome With MCC950 Improves Gut Health in Huntington's Disease Mice

doi: 10.1111/jnc.70419

Figure Lengend Snippet: Effects of MCC950 on motor function of HD and WT littermate control. Clasping score (a, b), rotarod performance (c, d), digigait (e, f). Data are displayed as mean ± SEM. Statistical analyses were performed using CLMMs (cumulative link mixed models) (a‐b), linear mixed model repeated measure analysis/generalised LMM repeated measure analysis (c, d), and linear mixed model (LMM)/generalised LMM (e, f) followed by Bonferroni post hoc adjustment with emmeans package in R. p value ( α ) = 0.05 and **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns= non‐significant n = 10–11 (male), n = 10 (female) for clasping score and rotarod; n = 8–10 (male), n = 9–10 (female) for digigait. Symbols in Figure , indicate the significance of post hoc comparisons as follows: *H2O (HD vs WT), #WT (H2O vs MCC950), $HD (H2O vs MCC950). Also, in Figure and , # symbol represents the significant interaction between treatment and age. One to four symbols in the graph represent p < 0.05 to p < 0.0001, respectively. HD, Huntington's disease; WT, wild type.

Techniques: Control

Journal: Journal of Neurochemistry

Article Title: Inhibition of the NLRP3 Inflammasome With MCC950 Improves Gut Health in Huntington's Disease Mice

doi: 10.1111/jnc.70419

Figure Lengend Snippet: Effects of MCC950 on short‐term memory and learning of HD and WT littermate control mice. Novel object recognition (NOR) test (a–c), Y‐maze test (d–g). Data are displayed as mean ± SEM. Statistical analyses were performed using linear mixed model (LMM)/generalised LMM (a–c), generalised linear model (d) and linear mixed model/generalised LMM repeated measure analysis (c, f, g) followed by Bonferroni post hoc adjustment with emmeans package in R. p value ( α ) = 0.05 and **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = non‐significant. n = 10–11 (male), n = 10 (female). HD, Huntington's disease; WT, wild type.

Techniques: Control

Journal: Journal of Neurochemistry

Article Title: Inhibition of the NLRP3 Inflammasome With MCC950 Improves Gut Health in Huntington's Disease Mice

doi: 10.1111/jnc.70419

Figure Lengend Snippet: Effects of MCC950 on longer‐term associative cognition and spatial memory of HD and WT littermate control mice (CFC test). Fear conditioning (a, b) and fear extinction (c, d). Data are displayed as mean ± SEM. Statistical analyses were performed using linear mixed model repeated measure analysis/generalised LMM repeated measure analysis (a–d) followed by Bonferroni post hoc adjustment with emmeans package in R. p ‐value ( α ) = 0.05 and ####,**** p < 0.0001, ###,*** p < 0.001, ##,** p < 0.01, #,* p < 0.05. n = 10–11 (male), n = 10 (female). HD, Huntington's disease; MCC, MCC950; WT, wild type.

Techniques: Control

Journal: Journal of Neurochemistry

Article Title: Inhibition of the NLRP3 Inflammasome With MCC950 Improves Gut Health in Huntington's Disease Mice

doi: 10.1111/jnc.70419

Figure Lengend Snippet: Effects of MCC950 on longer‐term associative cognition and spatial memory of HD and WT littermate control mice (MWM). Latency to reach the platform during training period of MWM test (a, b), distance travelled to reach the platform during training period of MWM test (c, d), time spent in each quadrant on day 6 of MWM test (e, f), latency to reach the platform on day 6 of MWM test (g), times spent in target quadrant on day 6 of MWM test (h), total distance travelled on day 6 of MWM test (i), velocity on day 6 of MWM test (j). Data are displayed as mean ± SEM. Statistical analyses were performed using linear mixed model repeated measure analysis/generalised LMM repeated measure analysis (a–f) and linear mixed model (LMM)/generalised LMM (g–j) followed by Bonferroni post hoc adjustment with emmeans package in R. p value ( α ) = 0.05 and ####,**** p < 0.0001, ###,*** p < 0.001, ##,** p < 0.01, #,* p < 0.05, ns= non‐significant. n = 8–11 (male), n = 7–10 (female). HD, Huntington's disease; MWM, Morris water maze; NE, northeast; NW, northwest; SE, southeast (target quadrant); SW, southwest; WT, wild type.

Techniques: Control

Journal: Journal of Neurochemistry

Article Title: Inhibition of the NLRP3 Inflammasome With MCC950 Improves Gut Health in Huntington's Disease Mice

doi: 10.1111/jnc.70419

Figure Lengend Snippet: Effects of MCC950 on the gut profile of HD and WT littermate control mice. Faecal water content (a, b), faecal consistency (Bristol stool score) (c, d), faecal output (e, f), gut transit time (GTT) (g, h), gut permeability (i, j). Data are displayed as mean ± SEM. Statistical analyses were performed using linear mixed model repeated measure analysis/generalised LMM repeated measure analysis (a, b, e–h), CLMMs (cumulative link mixed models) (c, d), linear mixed model (LMM)/generalised LMM (i, j) followed by Bonferroni post hoc adjustment with emmeans package in R. p ‐value ( α ) = 0.05 and ####,**** p < 0.0001, ###,*** p < 0.001, ##,** p < 0.01, #,* p < 0.05. n = 10–11 (male), n = 10 (female). HD, Huntington's disease; GTT, gut transit time; WT, wild type.

Techniques: Control, Permeability

Journal: Journal of Neurochemistry

Article Title: Inhibition of the NLRP3 Inflammasome With MCC950 Improves Gut Health in Huntington's Disease Mice

doi: 10.1111/jnc.70419

Figure Lengend Snippet: Effects of MCC950 on gut macroscopy of HD and WT littermate control mice. Cecal length (a, b), cecal weight (c, d), colon length (e, f), colon weight (g, h), colon weight to length ratio (i). Data are displayed as mean ± SEM. Statistical analyses were performed using linear mixed model (LMM)/generalised LMM followed by Bonferroni post hoc adjustment with emmeans package in R. p value ( α ) = 0.05 and **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = non‐significant. n = 9–11 (male), n = 9–10 (female). HD, Huntington's disease; WT, wild type.

Techniques: Control

Journal: Journal of Neurochemistry

Article Title: Inhibition of the NLRP3 Inflammasome With MCC950 Improves Gut Health in Huntington's Disease Mice

doi: 10.1111/jnc.70419

Figure Lengend Snippet: Effect of MCC950 on gut microbiome diversity of HD and WT littermate control mice. Comparison of community (Shannon alpha diversity) of gut microbiome stratified by sex under MCC950 or water for both WT and HD mice (a). PCA plot of microbial ASV based on Aitchison distance for Beta diversity (b). n = 8 (male), n = 8 (female). Box plots show the median (central line) and interquartile range (box); whiskers represent 1.5× the interquartile range; points represent individual animals. ASV, amplicon sequence variant; HD, Huntington's disease; PCA, principal component analysis; WT, wild type.

Techniques: Control, Comparison, Amplification, Sequencing, Variant Assay

Journal: Journal of Neurochemistry

Article Title: Inhibition of the NLRP3 Inflammasome With MCC950 Improves Gut Health in Huntington's Disease Mice

doi: 10.1111/jnc.70419

Figure Lengend Snippet: Effect of MCC950 on NLRP3, IL‐1β, Caspase‐1 expression in gut (proximal colon) of HD and WT littermate control mice. NLRP3 and IL‐1β (a), caspase‐1 (b, c). Data are displayed as mean ± SEM. Statistical analyses were performed using linear model (LM)/generalised LM followed by Bonferroni post hoc adjustment with emmeans package in R. p value ( α ) = 0.05 and **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = non‐significant. n = 5–7 (2 blots for males and 2 blots for females). After transferring the protein from gel to membrane, ponceau S staining was performed and then each membrane was split into two parts based on the size of the protein of interest. Upper part of the membrane was probed with NLRP3 antibody. While lower part of the membrane was first probed with IL‐1β followed by caspase‐1. Membrane was stripped using stripping buffer in between two experiments of IL‐1β and caspase‐1 detection. Representative image of ponceau S staining as loading control for both NLRP3 and IL‐1β (a), and caspase‐1 (b) is shown in NLRP3 and IL‐1β (a) panel only as it was derived from a single membrane and image was captured before probing with any antibody. Positive controls, poly I:C (spleen), BMDM: LPS + Nig. BMDM, Bone‐marrow‐derived macrophage; Cas 1 KO, caspase‐1 knock out; HD H 2 O, Huntington's disease water; HD MC, Huntington's disease MCC950, NL KO, NLRP3 knock out; IL‐1beta KO, Interleukin 1 beta knock out; IL‐1β/IL‐1beta, Interleukin 1 beta; LPS, Lipopolysaccharides; Nig, nigericin; WT H 2 O, wild type water; WT MC, wild type MCC950.

Techniques: Expressing, Control, Transferring, Membrane, Staining, Stripping Membranes, Derivative Assay, Knock-Out

(A) Representative F-VEP waveforms between the BE 24h, BE 24h + DMSO, and BE 24h + MCC950 groups, the horizontal grid is 50 ms and the vertical grid is 10 μV. (B-C) Quantitative analysis of P2 latency and N2-P2 amplitude (n = 20). (D) Representative F-VEP waveforms between the BE 28d, BE 28d + DMSO, and BE 28d + MCC950 groups. (E-F) Quantitative analysis of P2 latency and N2-P2 amplitude (n = 20). (G) Representative heatmap (left figure) and trajectory (right figure) visualization of mouse positions in different groups. (H-I) Quantitative analysis of stay time in dark areas in different groups (n = 12). All group information is shown in images. Error bars indicate mean ± SD. *** p < 0.001, ns p > 0.05.

Journal: PLOS One

Article Title: Impact of blast exposure on visual pathway: Mechanism exploration and novel diagnostic perspectives

doi: 10.1371/journal.pone.0344993

Figure Lengend Snippet: (A) Representative F-VEP waveforms between the BE 24h, BE 24h + DMSO, and BE 24h + MCC950 groups, the horizontal grid is 50 ms and the vertical grid is 10 μV. (B-C) Quantitative analysis of P2 latency and N2-P2 amplitude (n = 20). (D) Representative F-VEP waveforms between the BE 28d, BE 28d + DMSO, and BE 28d + MCC950 groups. (E-F) Quantitative analysis of P2 latency and N2-P2 amplitude (n = 20). (G) Representative heatmap (left figure) and trajectory (right figure) visualization of mouse positions in different groups. (H-I) Quantitative analysis of stay time in dark areas in different groups (n = 12). All group information is shown in images. Error bars indicate mean ± SD. *** p < 0.001, ns p > 0.05.

Article Snippet: MCC950 (HY-12815A, MCE, USA) was administered as previously described [ , ].

Techniques:

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mcc950 sodium (Selleck Chemicals)

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